Madidi (Spanish pronunciation: [maˈðiði]) is a national park in the upper Amazon river basin in Bolivia. Established in 1995,[2] it has an area of 18,958 square kilometres, and, along with the nearby protected (though not necessarily contiguous) areas Manuripi-Heath, Apolobamba, and (across the border in Peru) the Manu Biosphere Reserve, Madidi is part of one of the largest protected areas in the world.

Ranging from the glacier-covered peaks of the high Andes Mountains to the tropical rainforests of the Tuichi River, Madidi and its neighbors are recognized as one of the planet's most biologically diverse regions.[3] In particular, Madidi protects parts of the Bolivian Yungas and Bolivian montane dry forests ecoregions.

The Madidi National Park can be reached from Rurrenabaque if you cross the Beni River with the small passenger ferry over to San Buenaventura.

The local people who have migrated here from the Andean highlands speak the Quechua language. The cultures who find their origin here are the Tacana, the Mosete, the Tsimane, and the Ese Ejja, all of which have their own language which pertains to one language group.

Some ecolodges are found in and around the Madidi National Park. The oldest and best known is Chalalan Ecolodge in Chalalán on the Tuichi River, a successful community-based enterprise that generates significant economic benefits to indigenous communities (Malky et al., 2007).